FREe CANADA-WIDE EXPRESS SHipping on orders over $250 📦
PAYMENT BY INTERAC ONLY, Pay by CC soon
Reviews transferred over to Yotpo (over 140 reviews)
Retatrutide 30mg - 99.79% purity
🍁 Owned and operated by a cf veteran 🇨🇦
11 new coa rpts
Easter sale 15% off purchases over $250 (FCFS - while supplies last)
Coupon code: peptideparty

FREE CANADA-WIDE EXPRESS SHIPPING ON ORDERS OVER $250

Reviews transferred over to Yotpo (over 140 reviews)

🍁 Owned and operated by a CF Veteran 🇨🇦

Coupon Codes:

FREE Pfizer BW on Purchases over $500: blackdragon, 10% off: peptide party

Click Here

SALE ENDS TONIGHT (NO EXCEPTIONS), Reta 10mg $79, GH low quantities left

Pfizer bac water – In stock New Batch of G-H coming. RESTOCKED – Please double-check junk mail for emails

  • Home
  • Peptide Reconstitution Calculator

Free peptide calculator · Research use only

Peptide Calculatorreconstitution, concentration & U-100 syringe units

Enter the vial quantity in mg, the bacteriostatic water you added in mL, and a target quantity. The calculator returns concentration in mcg/mL, draw volume in mL, and the matching marking on a U-100 insulin syringe — instantly, free, with no signup.

Free · no signup Works on any vial in mg mg · mcg · mL · U-100 Arithmetic only — no dose advice

Enter your values

Step 1
Syringe capacity

Your result

Live
Draw to this marking
5.0units

= 0.050 mL on a U-100 (1.0 mL) insulin syringe

Concentration
5,000 mcg/mL
Per syringe unit
50.00 mcg
Draws per vial
40
Research use only. This peptide calculator performs arithmetic on values you enter. It does not recommend a quantity, establish a safe human dose, or replace a validated laboratory protocol. Verify every conversion against the specific vial label.
Equation 1 · Concentration

concentration = vial mg × 1,000 ÷ mL of water

Gives mcg per mL. This is the reconstitution step.

Equation 2 · Draw volume

volume = target mcg ÷ concentration

Gives millilitres. Enter mg and the calculator converts first.

Equation 3 · Syringe units

units = volume in mL × 100

Only true on a U-100 syringe, where 100 units = 1 mL.

Peptide reconstitution chart

Vial size × water volume, at a glance

The same arithmetic the calculator runs, laid out as a reference chart. Find your vial size down the left, the volume of bacteriostatic water you added across the top, and read off the concentration. No quantities are recommended anywhere in this table.

Concentration in mcg per mL. 1 mg = 1,000 mcg · 100 units = 1 mL on a U-100 syringe.
Vial+ 1 mL+ 2 mL+ 3 mL+ 5 mL+ 10 mL
2 mg2,0001,000667400200
5 mg5,0002,5001,6671,000500
10 mg10,0005,0003,3332,0001,000
15 mg15,0007,5005,0003,0001,500
20 mg20,00010,0006,6674,0002,000
30 mg30,00015,00010,0006,0003,000
50 mg50,00025,00016,66710,0005,000
100 mg100,00050,00033,33320,00010,000

Reading the highlighted cell: a 10 mg vial with 2 mL of bacteriostatic water gives 5,000 mcg/mL, so every syringe unit on a U-100 barrel holds 50 mcg.

mL to syringe units

Converting millilitres to insulin syringe units

Most confusion with a peptide calculator happens at this step. A U-100 insulin syringe is graduated so that 100 units equals exactly 1 mL — the units are a volume scale, not a measure of peptide mass.

Volume → U-100 units
VolumeU-100 units
0.01 mL1 unit
0.025 mL2.5 units
0.05 mL5 units
0.10 mL10 units
0.20 mL20 units
0.25 mL25 units
0.30 mL30 units
0.50 mL50 units
1.00 mL100 units
Mass conversion
MilligramsMicrograms
0.05 mg50 mcg
0.1 mg100 mcg
0.25 mg250 mcg
0.5 mg500 mcg
1 mg1,000 mcg
2.5 mg2,500 mcg
5 mg5,000 mcg
10 mg10,000 mcg
20 mg20,000 mcg

A unit is always the same volume. What changes is how much peptide sits inside that volume — which is set entirely by how much water you added at reconstitution.

Reconstitution method

How to reconstitute peptides, step by step

Reconstitution is the process of returning a lyophilised (freeze-dried) peptide powder to solution with a sterile diluent. The steps below describe general laboratory handling; follow your own validated protocol and the documentation supplied with the specific batch.

STEP 01

Let the vial reach room temperature

Bring the lyophilised vial up from cold storage before opening. Condensation on a cold stopper is avoidable contamination.

STEP 02

Read the label, not your memory

Record the labelled mass in mg from the vial in front of you. Two vials of the same compound can carry different fills.

STEP 03

Swab both stoppers

Wipe the peptide vial stopper and the bacteriostatic water stopper with an alcohol prep pad and let them dry.

STEP 04

Draw your chosen water volume

Decide the volume first — it sets the concentration for the life of the vial. Use the calculator above before you pierce anything.

STEP 05

Run the water down the glass wall

Angle the needle so the stream hits the side of the vial rather than jetting directly onto the powder pellet.

STEP 06

Swirl gently — never shake

Rotate the vial slowly until the solution is clear. Agitation and foaming place mechanical stress on peptide chains.

Once dissolved, the vial's concentration is fixed. Every later calculation on this page depends on the water volume recorded at this step, so write it on the vial.

Bacteriostatic water

How much bacteriostatic water should you add?

There is no universally correct volume — the amount you add is a measurement decision, not a potency decision. The vial holds the same total mass either way. What changes is how concentrated the solution is, and therefore how large or small each draw becomes on the syringe.

Red Leaf supplies the diluent itself: bacteriostatic water in 10 mL vials, and Pfizer bacteriostatic water 30 mL for multi-dose work. Both ship from Canada.

Less water · 10 mg + 1 mL
10,000 mcg/mL

A 250 mcg target lands at 2.5 units.

  • Very small draw volumes
  • Harder to measure precisely — a half-unit error is a large proportional error
  • Less total liquid to store
More water · 10 mg + 3 mL
3,333 mcg/mL

The same 250 mcg target lands at 7.5 units.

  • Larger, easier-to-read draw volumes
  • Small reading errors matter proportionally less
  • More liquid volume in the vial to store and use

Why bacteriostatic water rather than sterile water. Bacteriostatic water contains 0.9% benzyl alcohol as a preservative, which is what allows a multi-draw vial to be entered more than once. Plain sterile water contains no preservative and is intended for single use. Some protocols call for acetic acid or another specific reconstitution solution instead — that choice belongs to the protocol and the compound's documentation, not to a calculator.

Whatever diluent you use, the arithmetic on this page is identical: the calculator only cares about the volume you added, not what the liquid was.

Worked examples

Four calculations, start to finish

Each example runs all three equations in order so you can check your own working against it.

10 mg vial

+ 2 mL water · target 250 mcg

Concentration10 × 1,000 ÷ 2 = 5,000 mcg/mL
Draw volume250 ÷ 5,000 = 0.05 mL
On a U-100 syringe5 units

5 mg vial

+ 1 mL water · target 500 mcg

Concentration5 × 1,000 ÷ 1 = 5,000 mcg/mL
Draw volume500 ÷ 5,000 = 0.10 mL
On a U-100 syringe10 units

30 mg vial

+ 3 mL water · target 1 mg

Convert the target1 mg = 1,000 mcg
Concentration30 × 1,000 ÷ 3 = 10,000 mcg/mL
Draw volume1,000 ÷ 10,000 = 0.10 mL
On a U-100 syringe10 units

2 mg vial

+ 1 mL water · target 200 mcg

Concentration2 × 1,000 ÷ 1 = 2,000 mcg/mL
Draw volume200 ÷ 2,000 = 0.10 mL
On a U-100 syringe10 units

Notice examples two, three and four all land on 10 units with completely different vials. The unit marking describes volume — never mass.

Syringe reference

Which insulin syringe matches your draw

All three barrels below use the same U-100 scale — 100 units per mL. The difference is how far that scale runs and how finely it is graduated.

BarrelFull scaleTypical graduationBest suited to
0.3 mL30 units1 unitSmall draws under 30 units, where fine resolution matters most
0.5 mL50 units1 unitMid-range draws up to half a millilitre
1.0 mL100 units2 unitsLarger draws, dilute solutions, or drawing a full millilitre

U-100 and U-40 are not interchangeable. A U-40 syringe is graduated at 40 units per mL, so 10 units on a U-40 barrel is 0.25 mL — two and a half times the volume of 10 units on a U-100 barrel. This calculator assumes U-100 throughout, which is the standard scale on insulin syringes sold in Canada. Check the barrel before you rely on any unit figure.

Troubleshooting

Why your number disagrees with a chart

When a peptide calculator and a dosing chart give different answers, it is almost always one of these six things.

01

The mg / mcg toggle

Entering 250 with the unit set to mg instead of mcg inflates the target a thousandfold. This is by far the most common error.

02

A different water volume

Charts assume a specific reconstitution volume. If yours differs by even 1 mL, every figure below it changes.

03

U-40 syringe on a U-100 figure

Reading a U-100 unit count off a U-40 barrel gives 2.5× the intended volume.

04

Decimal placement

0.05 mL and 0.5 mL are one keystroke apart and an order of magnitude apart. Re-derive rather than re-reading.

05

A remembered vial size

Batches and product lines change fill weights. Read the vial in front of you every time.

06

Rounding at the wrong step

Round only at the final unit figure. Rounding concentration first compounds the error through both later equations.

Terminology

Peptide calculator glossary

Reconstitution
Returning a lyophilised powder to liquid form by adding a measured volume of sterile diluent.
Lyophilised
Freeze-dried. The state most research peptides ship in, because dry powder is far more stable than solution.
Diluent
The liquid added at reconstitution — commonly bacteriostatic water, sometimes sterile water or another specified solution.
Bacteriostatic water
Sterile water containing 0.9% benzyl alcohol, which inhibits bacterial growth and permits multiple entries into a vial.
Concentration
How much peptide sits in each millilitre of solution, expressed here as mcg/mL. Set once, at reconstitution.
mg
Milligram. The unit vials are almost always labelled in.
mcg
Microgram. One thousandth of a milligram — 1 mg = 1,000 mcg.
mL
Millilitre. The unit of liquid volume used for both the diluent and the draw.
U-100
An insulin syringe scale where 100 units equals 1 mL. A volume scale, not a mass scale.
IU
International unit — a biological-activity measure used on some vials, notably growth hormone. Converts to mg only via the compound's own stated factor.
COA
Certificate of analysis. Third-party laboratory documentation of identity and purity for a specific batch.
RUO
Research use only. Material supplied for laboratory research, not for human or veterinary use.

Same tool, different search

Which peptide calculator are you looking for?

These names all describe the same three conversions. This page handles every one of them.

Peptide calculator

The general term. Converts mass, volume, concentration and U-100 markings in a single pass.

The tool above

Peptide reconstitution calculator

Starts from vial mass and total liquid volume to find the concentration of the reconstituted vial.

Equation 1

Peptide dosage calculator

Converts a quantity you enter into a draw volume. It does not select or recommend any quantity.

Equation 2

Peptide dosing calculator

Same arithmetic under another name. The tool converts; the protocol decides.

Equation 2

Peptide mixing calculator

Answers the "how much water do I add" side by showing what each volume does to concentration.

Chart above

Reconstitution calculator for BPC-157, PT-141, MOTS-C, GLP-1 or HGH vials

Compound-agnostic. Any vial labelled in mg follows identical arithmetic.

Equations 1–3

Quick answers

Peptide calculator FAQ

The questions people search alongside this tool, grouped by what they are actually trying to work out.

The arithmetic

How do you calculate peptide concentration?
Divide the vial quantity by the volume of bacteriostatic water added. A 10 mg vial reconstituted with 2 mL gives 5 mg/mL, which is 5,000 mcg/mL. The calculator recomputes this the instant you change either number.
How do I convert mL to units on an insulin syringe?
Multiply the volume in mL by 100. A U-100 insulin syringe is graduated so that 100 units equals 1 mL, so 0.05 mL is 5 units and 0.25 mL is 25 units.
How many mL is one unit on an insulin syringe?
On a U-100 syringe, one unit is 0.01 mL. On a U-40 syringe it is 0.025 mL — which is why the two scales must never be used interchangeably.
How many mcg are in a mg?
One milligram is 1,000 micrograms. A 10 mg vial therefore contains 10,000 mcg of peptide in total, however much water you later add to it.
How do I work out how many draws a vial holds?
Divide the total vial mass by your target quantity. A 10 mg vial against a 250 mcg target gives 40 draws, regardless of the water volume — adding more water changes the size of each draw, not how many the vial contains.
Why does my number differ from a peptide dosing chart PDF?
Usually a unit or volume mismatch. Charts are built around one assumed reconstitution volume, so if yours differs the whole column shifts. Check the mg/mcg toggle first, then the water volume, then re-derive rather than re-reading.

Reconstitution

How much bacteriostatic water should I add to a vial?
There is no single correct volume. The amount you add sets the concentration and therefore the size of every subsequent draw — more water gives larger, easier-to-measure volumes; less water gives smaller ones. The vial contains the same total mass either way. Use the chart above to see how each combination lands before you open anything.
Can I reconstitute peptides with saline or plain sterile water?
Both are used in laboratory settings, but neither contains a preservative, so they suit single-entry work rather than a vial you will return to. Bacteriostatic water contains 0.9% benzyl alcohol for that reason. Some compounds specify a different reconstitution solution entirely — the compound's documentation governs that choice, not a calculator.
Should I shake the vial to dissolve the powder?
No. Direct the diluent down the inside wall of the vial rather than onto the powder, then swirl slowly until clear. Shaking foams the solution and places mechanical stress on the peptide chains.
How long does a reconstituted peptide last in the fridge?
Stability after reconstitution varies by compound, diluent, temperature and light exposure, and it is documented per compound rather than by a universal rule. Reconstituted material is generally kept refrigerated and protected from light; consult the batch documentation for the specific compound rather than applying a blanket figure.
What should I write on the vial after reconstituting?
The water volume you added and the date. Concentration is impossible to recover later from the vial alone, and every calculation on this page depends on that number.

Scope and limits

Is this a peptide dosage calculator?

It performs the arithmetic people mean when they search that phrase, but it does not select or recommend a quantity for anyone. You supply the target value; the tool converts it into volume and syringe units.

It is not medical advice and does not establish a safe human dose.

Does the calculator work for BPC-157, PT-141, MOTS-C, GLP-1 or HGH vials?
Yes — the arithmetic is compound-agnostic. Any vial labelled in milligrams and reconstituted with a measured volume in millilitres follows exactly the same three equations, whatever the compound.
What about vials labelled in IU rather than mg?
Enter the vial in mg. International units measure biological activity, not mass, and the conversion factor is specific to the compound — for somatropin it is commonly stated as roughly 3 IU per mg, but you should take the factor from the specific product's own documentation rather than assuming it.
What if the draw exceeds my syringe capacity?
The calculator flags it and names a barrel that would hold the volume. If the draw exceeds a full 1.0 mL, the solution is too concentrated for a single draw — reconstituting with more water lowers the concentration and brings the volume back into range.
Is there a peptide calculator app to download?
This one runs in the browser with no install, no account and no data leaving your device — the arithmetic happens locally as you type. Save the page to your home screen if you want it one tap away.
Is the calculator free?
Yes, entirely. There is no signup, no usage limit and no requirement to buy anything.
Is this tool intended for human or animal use?
No. Red Leaf Research Labs supplies materials for laboratory research use only. This calculator is a unit-conversion tool for that context and is not intended to guide use in humans or animals.

Red Leaf Research Labs

Calculate clearly. Verify confidently.

Every vial we ship carries third-party Janoshik analysis with a verify link, so the mass on the label is the number your calculation actually rests on.

Shop research peptides Testing & COAs Bacteriostatic water
These materials are not approved by Health Canada for cosmetic, therapeutic or veterinary use. Sold for laboratory research use only. This calculator performs arithmetic on user-entered values, does not provide medical advice, and does not establish a safe quantity for any purpose.

Post this calculator anywhere you like

Free to use and no permission needed. Drop this into a forum post, a blog, or your own website and the calculator runs there. The only condition is that you keep the credit line underneath it.

Researcher in a red Red Leaf Research shirt using a peptide reconstitution calculator on a tablet
Research Peptides Canada - Red Leaf Research

Disclaimer

You must be 18 or 19 years of age depending on the age of majority in your province and a licensed research professional

All products are sold in powder (lyophilized) form and require reconstitution with a suitable diluent for research purposes only. No dosing instructions are provided. We adhere to all provincial and federal laws in Canada around Research Only Chemical sales. We are not a pharmacy and/or compounding facility, nor do we promote or provide any advice for human or animal consumption.

By clicking ”I Agree” you confirm that you have read and accepted the Terms and Conditions stated above

I Agree with the Terms
0